Journal: Stem Cell Research & Therapy

Article Title: Vasoactive intestinal peptide promotes secretory differentiation and mitigates radiation-induced intestinal injury

doi: 10.1186/s13287-024-03958-z

Figure Lengend Snippet: VIP mitigates irradiation-induced injury in vivo. A Experimental outline and treatment scheme for VIP or water (IR control) administration in mice following acute abdominal irradiation, compared with sham-irradiated untreated (Sham Control) mice. B , ELISA analysis of VIP levels in the intestinal wall on day 6 following acute abdominal irradiation. n = 5 (Sham Control), n = 7 (IR Control). C , ELISA analysis of TNF-alpha levels in the intestinal wall of sham-irradiated and irradiated mice in the absence or presence of VIP. n = 7 (Sham Control), n = 6 (IR Control; IR VIP). D Pathological scoring of irradiated mouse tissue in the absence or presence of VIP and representative H&E images (Scale bars = 100 µm), n = 5 per group. E, Immunofluorescent analysis of LYZ1 staining in jejunal tissue from irradiated mice in the absence or presence of VIP (Scale bars = 100 µm). n = 7 (Sham Control; IR Control), n = 8 (IR VIP). * p < 0.05, ** p < 0.01, *** p < 0.001. Data are shown as means ± SEM, n = number of biological replicates. Statistical analysis was performed by Student’s t -test in ( B , D ) and one-way ANOVA with Tukey's multiple comparisons test in ( C , E )

Article Snippet: VIP and TNF-alpha levels were measured using Mouse VIP (Competitive EIA) ELISA Kit (LSBio, #LS-F4059) and Mouse TNF Alpha (Sandwich ELISA) ELISA Kit (LSBio, #LS-F5192), respectively, according to the manufacturer’s instructions.

Techniques: Irradiation, In Vivo, Control, Enzyme-linked Immunosorbent Assay, Staining